Free Seminar Professor Yvonne Perrie
Professor Yvonne Perrie
Head of School of Pharmacy and Professor of Drug Delivery
Aston University, Birmingham UK
Title: Continuous manufacturing of liposomes using microfluidics for drug and vaccine delivery
Time: 11 am - 12 pm
Venue: Lecture Theatre 2
A key limitation in the wider application of liposomes is their costly and complex manufacturing needs. To address this and develop new methods for liposome and nanoparticle production we have been investigating the application of microfluidics for continuous manufacture of liposomes. Recent advances in lab-on-a-chip based technologies have already led to microfluidic-based methodologies in drug development. Microfluidics-based methods (which exploit controlled mixing of streams in micro-sized channels) have been described for the manufacture of lipid-based nanoparticles. Those systems achieve a tight control of the mixing rates, requiring lower liquid volumes and facilitating process development by reducing time and development costs. The systems are designed with the option of high-throughput and continuous manufacturing and can be considered less harsh compared to conventional methods of nanoparticle manufacturing.
To investigate the formulation of liposomes using microfluidics small unilamellar vesicles were manufactured using the mixing platform NanoAssemblr™ (Precision NanoSystems Inc.), based on a staggered herringbone micromixer. Lipids (Egg Phosphatidylcholine (PC) and Cholesterol, 16:4 molar ratio) in solvent (Ethanol) and aqueous buffer (TRIS 10mM, pH 7.2) were injected into separate chamber inlets followed by rapid mixing and liposome synthesis by a nanoprecipitation process. Propofol (2,6-Bis(isopropyl)phenol) was dissolved with the lipids in ethanol and liposome formation and encapsulation of the drug were performed simultaneously using the micromixer method. Liposomes were dialyzed to remove any unentrapped drug and solvent residues. Characterisation of liposomes included size, zeta potential (zp) and polydispersity (pdi) determination (Zetasizer Nano-ZS, Malvern). Encapsulated propofol was quantified by a validated HPLC method (Luna 5µ C18, Phenomenex).
Our results demonstrate the ability of merging liposome manufacturing and drug encapsulation in a single process step with demonstrated scalability. The lab-on-a-chip based method emphasizes on the flexibility and ease of applying microfluidics for the manufacturing of drug-loaded liposomes.
Bio: Professor Yvonne Perrie is the Head of Pharmacy and Chair in Drug Delivery within Aston Pharmacy School, Aston University, Birmingham, UK. Yvonne’s research is multi-disciplinary and is focused on the development of particulate carrier systems to facilitate the delivery of drugs and vaccines and thus provide practical solutions for current healthcare problems. Yvonne has published approx. 100 peer reviewed manuscripts and 3 textbooks., Yvonne’s work has also been translated into 5 patents, one of which played an integral role in the development of the DNA vaccine platform for Lipoxen Technologies Ltd. Externally Yvonne has numerous roles: she is a Director-at-large for the Controlled Release Society (CRS) and Editor of the Controlled Release Society Newsletter. Yvonne was previously the Chair for the UK and Ireland Controlled Release Society and Chair of the Board of Scientific Advisors for CRS. She is Editor in Chief of the Journal of Liposome Research and Pharmaceutics and Associate Editor for the Journal of Drug Targeting and the Journal of Pharmacy and Pharmacology.